Initial inspection of desktop sterilizer and factors affecting the sterilization effect of desktop sterilizer
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Summary: Benchtop sterilizers are widely used in medical applications; their primary function is sterilization. When using a sterilizer for the first time, a preliminary inspection is necessary to prevent operational errors. Below, we will examine the initial inspection of a benchtop sterilizer and the factors influencing its sterilization effectiveness.
Desktop sterilizers are widely used in medical applications; their primary function is sterilization and disinfection. When using a sterilizer for the first time, it's crucial to inspect it to prevent operational errors. Let's examine the initial inspection of a desktop sterilizer and the factors affecting its sterilization effectiveness.
I. Initial inspection of a desktop sterilizer is necessary. The specific checks are as follows:
Simple safety checks for non-automatic desktop sterilizers:
(1) Manually open the safety valve to check if it functions correctly.
(2) During heating, check if the pressure gauge needle's upward movement is obstructed. If not obstructed, it can be used normally.
Simple safety checks for automatic desktop sterilizers
(1) Manually open the safety valve to check if it functions correctly.
(2) When adding water, observe whether the water level indicator shows a normal water level.
(3) After removing cold air, observe the difference between the temperature on the display screen and the temperature indicated by the pressure gauge. Normally, the pressure gauge temperature is slightly higher than the display screen temperature. If the pressure difference does not exceed 3℃, it can be used normally.
II. Factors Affecting the Sterilization Effectiveness of Desktop Sterilizers
(1) Water: Excessive water temperature may alter the predetermined vacuum; the water temperature should be as low as possible. The water used in the sterilizer should meet the required water quality standards, with a temperature not exceeding 15℃. The water hardness should be between 0.7~2.0 mmol/L. Hardness values outside this range may lead to problems such as scaling and corrosion, shortening the lifespan of the desktop sterilizer. The water used should be filtered, and the sterilizer body should be kept clean.
(2) The desktop sterilizer should use saturated steam with a dryness factor of no less than 0.9, i.e., the steam moisture content does not exceed 10%, and under a metallic load, the dryness factor should be no less than 0.95, maintaining a linear relationship between temperature and pressure.
(3) Sterilization Time: Sterilization time refers to the time required for sterilization after the sterilization chamber reaches the specified temperature during the sterilization process.
(4) During operation, pay attention to the steam injection speed and pressure, generally maintaining a synchronous increase in pressure and temperature.
Let's look at the precautions for sterilizing liquid substances using an autoclave. In practice, liquid sterilization is often required. While desktop sterilizers are designed to sterilize bottled liquids, unexpected situations can occur. In an experiment, after sterilizing glass bottles, the liquid inside decreased by 1/3. Using plastic film to seal the bottles, if the sealing film breaks in the middle, the liquid in the bottle will still decrease by one-third. When sterilizing liquid substances, it is important to note that at the end of the sterilization, depressurization should not be too fast; adjust the exhaust valve to a smaller setting, maintaining natural depressurization and the system's original balance to prevent liquid reduction or splashing.
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