How to use and precautions for a desktop sterilizer
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Summary: A tabletop sterilizer is a high-pressure container that raises the boiling point of water above 100℃ under pressure higher than normal atmospheric pressure to sterilize liquids or instruments. It is suitable for sterilizing medical instruments, glassware, and solution media in medical and health institutions and scientific research institutions. Utilizing the effect of gravity displacement, steam is discharged from top to bottom within the sterilizer, and cold air is discharged from the bottom vent. The discharged cold air is replaced with saturated steam, and the latent heat released by the steam is used to sterilize the items. Below, we will look at the usage and precautions for a tabletop sterilizer:
A tabletop sterilizer is a high-pressure container that raises the boiling point of water above 100℃ under pressure higher than atmospheric pressure to sterilize liquids or instruments. It is suitable for sterilizing medical instruments, glassware, and solution media in medical and scientific research institutions. Using the effect of gravity displacement, steam is discharged from top to bottom within the sterilizer, and cold air is discharged from the bottom vent. The discharged cold air is replaced with saturated steam, and the latent heat released by the steam is used to sterilize the items. Below, we will look at the usage and precautions for tabletop sterilizers:
I. How to Use a Tabletop Sterilizer
(1) Add an appropriate amount of water to the sterilizer, place the items to be sterilized in the sterilizer, cover the lid, and tighten the screws symmetrically.
(2) Heat the sterilizer to generate steam. When the pressure gauge pointer reaches 33.78kpa, open the exhaust valve to exhaust the cold air. The pressure gauge pointer will drop, and when the pointer drops to zero, close the exhaust valve.
(3) Continue heating the steam sterilizer, and the pressure will rise again. When the pressure inside the sterilizer rises to the required pressure, heat it according to the characteristics of the sterilized items, maintain the steam pressure for a certain time, then turn off the sterilizer or power source, let it cool naturally, then slowly open the exhaust valve to release the remaining air, and then you can open it to retrieve the items.
II. Precautions for Tabletop Sterilizers
(1) Items to be sterilized should not be placed too tightly.
(2) Cold air must be fully exhausted; otherwise, the sterilizer temperature will not reach the specified temperature, affecting the sterilization effect.
(3) After sterilization, do not release pressure; otherwise, the liquid in the bottle will boil violently, the bottle stopper will pop out, causing spillage or even container rupture. The lid can only be opened when the pressure inside the sterilizer is reduced to equal atmospheric pressure.
(4) Wrap the cotton plugs of test tubes or bottles with oiled paper or kraft paper to prevent condensation from entering.
(5) To ensure the sterilization effect, the sterilization effect should be checked regularly. A common method is to place sulfur powder (melting point 115℃) or benzoic acid (melting point 120℃) in a test tube and then perform a sterilization test. If the above substances melt, it indicates that the temperature inside the pressure cooker has reached the requirement, and the sterilization effect is reliable. You can also paste adhesive tape (with a temperature-sensitive indicator) used to test the sterilization effect on the outer packaging of the item to be sterilized. If the indicator on the adhesive tape changes color, the sterilization effect is reliable.
III. Applications of Tabletop Sterilizers
As a commonly used sterilization method, treatment at 101.33kPa for 15-20min can achieve the purpose of sterilizing items. All items that can withstand high temperatures and humidity, such as ordinary culture media, physiological saline, clothing, gauze, and glass equipment, can be sterilized using this method.
Medical tabletop sterilizers have a wide range of uses and are suitable for medical and health institutions, beauty medical institutions, etc.; for sterilizing medical instruments, dressings, glassware, and solution culture media, it is a commonly used sterilization method in microbiological experiments. This sterilization method is based on the principle that the boiling point of water increases with increasing steam pressure.
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